User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/22: Difference between revisions
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The second experiment used an excitation of 370 and scanned from 390-540nm. | The second experiment used an excitation of 370 and scanned from 390-540nm. | ||
stock solution for tomorrow | |||
#tryptophan | |||
## 3.01 mg | |||
## 10.0 ml volumetric flask | |||
## 204.23 g/mol | |||
## 1.47 mM | |||
==Notes== | ==Notes== | ||
Revision as of 12:33, 22 September 2016
 Biomaterials Design Lab
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ObjectiveRunning fluorescence spectra of AuCl3 at 80 C and pH 4. Description
DataI ran an excitation spectrum (looking for emission at 430, which seems to be the absorption maxima that we see in our fluorescence spectra for protein AuNP synthesis). I didn't see much outside of the water raman peak. I am going to run 2 time-based emission spectra with excitation at 290 (what we excite with in our syntheses) and excitation at 370 (which should simulate energy transfer from W* to Au3+. The first experiment excited at 290 nm (10nm slit width) and measured the emission from 310-540nm (100 nm/min and 10nm slit width). These scan speeds and slit widths were used throughout. The spectra for the 290 excitation stayed featureless the entire 3 hours. I took a UV-vis after the reaction. Noticeably, there were no bubbles present in the sample, which we often see during AuNP synthesis. These could just be due to water evaporation. But we are unsure of their causes. I took a UV Vis spectrum before the second fluorescence measurement. The second experiment used an excitation of 370 and scanned from 390-540nm.
NotesThis area is for any observations or conclusions that you would like to note.
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