User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/22: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Data== | ==Data== | ||
* | I ran an excitation spectrum (looking for emission at 430, which seems to be the absorption maxima that we see in our fluorescence spectra for protein AuNP synthesis). I didn't see much outside of the water raman peak. I am going to run 2 time-based emission spectra with excitation at 290 (what we excite with in our syntheses) and excitation at 370 (which should simulate energy transfer from W* to Au3+. | ||
[[Image:20160922 mrh AuCl3 fluorescenceexcitation.png]] | |||
[[Image:20160922 mrh AuCl3 fluorescenceemission.png]] | |||
The first experiment excited at 290 nm (10nm slit width) and measured the emission from 310-540nm (100 nm/min and 10nm slit width). These scan speeds and slit widths were used throughout. | |||
The spectra for the 290 excitation stayed featureless the entire 3 hours. I took a UV-vis after the reaction. Noticeably, there were no bubbles present in the sample, which we often see during AuNP synthesis. These could just be due to water evaporation. But we are unsure of their causes. | |||
[[Image:20160922 mrh AuCl3 pH4 80C emission290ex.png]] | |||
[[Image:20160922 mrh AuCl3 pH4 80C Integrated290ex.png]] | |||
I took a UV Vis spectrum before the second fluorescence measurement. | |||
The second experiment used an excitation of 370 and scanned from 390-540nm. | |||
[[Image:20160922 mrh AuCl3 pH4 80C emission370ex.png]] | |||
[[Image:20160922 mrh AuCl3 pH4 80C Integrated370ex.png]] | |||
stock solution for tomorrow | |||
#tryptophan | |||
## 3.01 mg | |||
## 10.0 ml volumetric flask | |||
## 204.23 g/mol | |||
## 1.47 mM | |||
==Notes== | ==Notes== |
Latest revision as of 02:02, 27 September 2017
Biomaterials Design Lab | Main project page Previous entry Next entry |
ObjectiveRunning fluorescence spectra of AuCl3 at 80 C and pH 4. Description
DataI ran an excitation spectrum (looking for emission at 430, which seems to be the absorption maxima that we see in our fluorescence spectra for protein AuNP synthesis). I didn't see much outside of the water raman peak. I am going to run 2 time-based emission spectra with excitation at 290 (what we excite with in our syntheses) and excitation at 370 (which should simulate energy transfer from W* to Au3+. The first experiment excited at 290 nm (10nm slit width) and measured the emission from 310-540nm (100 nm/min and 10nm slit width). These scan speeds and slit widths were used throughout. The spectra for the 290 excitation stayed featureless the entire 3 hours. I took a UV-vis after the reaction. Noticeably, there were no bubbles present in the sample, which we often see during AuNP synthesis. These could just be due to water evaporation. But we are unsure of their causes. I took a UV Vis spectrum before the second fluorescence measurement. The second experiment used an excitation of 370 and scanned from 390-540nm. stock solution for tomorrow
NotesThis area is for any observations or conclusions that you would like to note.
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