User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/02: Difference between revisions
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4 scans from the first 10 minutes of measurements | 4 scans from the first 10 minutes of measurements. (Note: I highlight these individual spectrum because these are the spectra that correspond to the changes that are observed in the emission maximum versus time plot and the emission intensity versus time plot.) | ||
[[Image:20160902 mrh Lysozyme firstscans.png|500px]] | [[Image:20160902 mrh Lysozyme firstscans.png|500px]] |
Revision as of 08:11, 5 September 2016
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ObjectiveMeasure lysozyme unfolding at pH 4 and 80 °C with no gold present. This is a complimentary measurement for here and here. Descriptionmeasurement solution
(second sample)
(third sample)
DataThe first two samples were too concentrated and saturated the detector. I meant to take data every 5 minutes by took a sample at 3 minutes by mistake. It is labeled lysozyme_ph4_80C_05. The actual 5 minute sample is labeled as lysozyme_ph4_80C_05b. From then, I keep the 1 sample every 5 min pace. So I have samples for: 0, 3, 5, 10, 15, 20, etc.
NotesSpecial notes about fluorescence and the measurements we're taking.
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