User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/02: Difference between revisions
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Change in max wavelength as a function of time (signals the local environment of the tryptophan residues) | Change in max wavelength as a function of time (signals the local environment of the tryptophan residues) Note that the wavelength of emission maximum changes from 352 nm to 362 nm. Any changes to much higher wavelengths (that you WILL see in your spectra) are likely due to energy transfer to nearby energy accepting chemicals. | ||
[[Image:20160902 mrh Lysozyme pH4 emissionmax.png|500px]] | [[Image:20160902 mrh Lysozyme pH4 emissionmax.png|500px]] |
Revision as of 08:09, 5 September 2016
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ObjectiveMeasure lysozyme unfolding at pH 4 and 80 °C with no gold present. This is a complimentary measurement for here and here. Descriptionmeasurement solution
(second sample)
(third sample)
DataThe first two samples were too concentrated and saturated the detector. I meant to take data every 5 minutes by took a sample at 3 minutes by mistake. It is labeled lysozyme_ph4_80C_05. The actual 5 minute sample is labeled as lysozyme_ph4_80C_05b. From then, I keep the 1 sample every 5 min pace. So I have samples for: 0, 3, 5, 10, 15, 20, etc.
NotesSpecial notes about fluorescence and the measurements we're taking.
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