Objective
Remaking AuNP lysozyme samples for Hannah and Cosette. In their synthesis from yesterday (lab notebooks here and here). The fault here was mine in that I gave Hannah my calculation spreadsheet from when I made BSA-AuNPs and did not tell Hannah that we needed a different concentration of lysozyme than BSA. For BSA the ratio of Au:BSA to make AuNPs is 80 (with an Au concentration of 0.25). For lysozyme this ratio is 20. With an Au concentration of 250 μM, that puts the lysozyme concentration at 12.5μM. Predictably, all of their samples resulted in fibers.
Description
- Add experimental record here. Include what, how, and why...
Data
stock solutions
- AuCl3 stock A (from 8/30)
- mass = 10.13 mg
- 10.0 mL volumetric flask
- concentration = 3.34 mM
- AuCl3 stock B (from 8/31)
- mass = 12.6 mg
- 10.0 mL volumetric flask
- concentration = 4.17 mM
- Fructose (from 8/30)
- mass = 23.48 mg
- 10.0 mL volumetric flask
- concentration = 13.0 mM
- Lysozyme stock 1 (from 8/31)
- mass = 8.88 mg
- 10.0 mL volumetric flask
- concentration = 62.1 μM
- Lysozyme stock 2 (made today)
- mass = 21.92 mg
- 10.0 mL volumetric flask
- concentration = 153 μM
0 mM Fructose
pH
|
μL of 1 mM HClstock
|
μL of 1 mM NaOHstock
|
μL of 1 M NaOHstock
|
μL of AuCl3stock
|
μL of Fructosestock
|
μL of BSAstock
|
μL of Water
|
4 |
500 |
0 |
0 |
300B |
0 |
10061 |
3194
|
5 |
50 |
0 |
0 |
300B |
0 |
10061 |
3644
|
6 |
5 |
0 |
0 |
300B |
0 |
10061 |
3689
|
7 |
0 |
0 |
0 |
300B |
0 |
10061 |
3694
|
8 |
0 |
5 |
0 |
300B |
0 |
4082 |
4287
|
9 |
0 |
50 |
0 |
300B |
0 |
4082 |
4242
|
10 |
0 |
500 |
0 |
300B |
0 |
4082 |
3792
|
11 |
0 |
0 |
5 |
300B |
0 |
4082 |
4287
|
12 |
0 |
0 |
50 |
300B |
0 |
4082 |
4242
|
0.0625 mM Fructose
pH
|
μL of 1 mM HClstock
|
μL of 1 mM NaOHstock
|
μL of 1 M NaOHstock
|
μL of AuCl3stock
|
μL of Fructosestock
|
μL of BSAstock
|
μL of Water
|
4 |
500 |
0 |
0 |
300B |
24 |
4082 |
3768
|
5 |
50 |
0 |
0 |
300B |
24 |
4082 |
4218
|
6 |
5 |
0 |
0 |
300B |
24 |
4082 |
4263
|
7 |
0 |
0 |
0 |
300B |
24 |
4082 |
4268
|
8 |
0 |
5 |
0 |
300B |
24 |
4082 |
4263
|
9 |
0 |
50 |
0 |
374A |
24 |
4082 |
4144
|
10 |
0 |
500 |
0 |
374A |
24 |
4082 |
3694
|
11 |
0 |
0 |
5 |
374A |
24 |
4082 |
4189
|
12 |
0 |
0 |
50 |
374A |
24 |
4082 |
4144
|
Notes
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