User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2015/10/06: Difference between revisions

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==Objective==
==Objective==
Learn how to maintain an OpenWetWare Notebook.
# Roberta, Maxi, and Asya are running an experiment on the Ocean Optics spectrometer today. I will advise you of some changes to the procedure.
# Nouf, Loukas, and Melvin and Sydney, Andrew, and Matt will measure protease kinetics with the Bradford Assay using 1uM of protease. I will advise you of slight changes to the procedure.
# Jamie, Nicole, and Benjamin and Michael and Becky will measure protease kinetics with the Fluorescence Assay using 1uM of protease. I will advise you of slight changes to the procedure.
 
I think that we've been centrifuging our samples at too fast a speed. I checked through Paul and Monika's notebooks. They centrifuged their samples at 300 rpm. I think that, by spinning our samples so fast, we may be compromising them for our other measurements. So, I want to try going a little slower.
 
I made new samples last [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2015/10/02|Friday]].


==Description==
==Description==

Revision as of 17:41, 5 October 2015

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Objective

  1. Roberta, Maxi, and Asya are running an experiment on the Ocean Optics spectrometer today. I will advise you of some changes to the procedure.
  2. Nouf, Loukas, and Melvin and Sydney, Andrew, and Matt will measure protease kinetics with the Bradford Assay using 1uM of protease. I will advise you of slight changes to the procedure.
  3. Jamie, Nicole, and Benjamin and Michael and Becky will measure protease kinetics with the Fluorescence Assay using 1uM of protease. I will advise you of slight changes to the procedure.

I think that we've been centrifuging our samples at too fast a speed. I checked through Paul and Monika's notebooks. They centrifuged their samples at 300 rpm. I think that, by spinning our samples so fast, we may be compromising them for our other measurements. So, I want to try going a little slower.

I made new samples last Friday.

Description

  1. Add experimental record here. Include what, how, and why...

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.