User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2015/10/06: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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I made new samples last [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2015/10/02|Friday]]. | I made new samples last [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2015/10/02|Friday]]. | ||
==Bradford Analysis of Protease Degradation== | ==Bradford Analysis of Protease Degradation== | ||
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## Record the UV-Vis spectrum from 400-800 nm | ## Record the UV-Vis spectrum from 400-800 nm | ||
# Using the Bradford standardization curve and your data from the 0 fiber sample, determine the concentration of released protein in your solution. | # Using the Bradford standardization curve and your data from the 0 fiber sample, determine the concentration of released protein in your solution. | ||
==Fluorescence Analysis of Protease Degradation== | ==Fluorescence Analysis of Protease Degradation== | ||
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## Excitation at 390 nm | ## Excitation at 390 nm | ||
## Emission from 400 to 650 nm | ## Emission from 400 to 650 nm | ||
==Description== | ==Description== |
Latest revision as of 01:14, 27 September 2017
Biomaterials Design Lab | Main project page Previous entry Next entry |
Objective
I think that we've been centrifuging our samples at too fast a speed. I checked through Paul and Monika's notebooks. They centrifuged their samples at 300 rpm. I think that, by spinning our samples so fast, we may be compromising them for our other measurements. So, I want to try going a little slower. I made new samples last Friday. Bradford Analysis of Protease DegradationNote: You will be making samples (and blanks) for analysis at different points of time. So plan wisely. All samples will be incubated in eppendorf tubes, which will be placed in the 37C water bath on the shaker. Samples should all contain the same concentration of materials. You should make samples for measurements at 10 minutes, 15 minutes, 30 minutes, 45 minutes, 1 hr, 1.5 hr, 2 hr. (Note, this is for the first time you do this. As you get better, I will expect you to get more measurements done).
Fluorescence Analysis of Protease DegradationWe are using the Pierce quantitative fluorometric peptide assay. (product link here) Note: You will be making samples (and blanks) for analysis at different points of time. So plan wisely. All samples will be incubated in eppendorf tubes, which will be placed in the 37C water bath on the shaker. Samples should all contain the same concentration of materials. You should make samples for measurements at 10 minutes, 15 minutes, 30 minutes, 45 minutes, 1 hr, 1.5 hr, 2 hr. (Note, this is for the first time you do this. As you get better, I will expect you to get more measurements done).
Description
Data
NotesThis area is for any observations or conclusions that you would like to note.
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