User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2015/09/02: Difference between revisions

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==Description==
==Description==
<u>Target Concentrations</u>
<u>Target Concentrations</u>
Based on the total reaction volume that the class will be using and the final concentrations that will be in each reaction volume, I have determined that the following  HAuCl<sub>4</sub>·H<sub>2</sub>O and Lysozyme masses are appropriate for today's stock solutions.


<u>Stock solutions</u>
<u>Stock solutions</u>
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<u>Actual Concentrations</u>
<u>Actual Concentrations</u>
The actual masses are different from the target masses. The concentrations shown below reflect the actual concentrations made for the stock solutions. I used a 50 mL volumetric flask to make the gold stock solution and a 100 mL volumetric flask to make the protein solution. In each case I used HPLC-grade water.


<u>Stock solutions</u>
<u>Stock solutions</u>

Revision as of 06:27, 2 September 2015

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Objective

Prepping stock solutions for use in making AuNP fibers with lysozyme for other researchers to use.

Description

Target Concentrations

Based on the total reaction volume that the class will be using and the final concentrations that will be in each reaction volume, I have determined that the following HAuCl4·H2O and Lysozyme masses are appropriate for today's stock solutions.

Stock solutions

  1. HAuCl4·H2O
    1. 2.5 mM
    2. MW = 339.79 g/mol
    3. Volume = 50 mL
    4. 42.4 mg
  2. Lysozyme
    1. 20 μM
    2. MW = 14307 g/mol
    3. Volume = 100 mL
    4. 28.6 mg

Actual Concentrations

The actual masses are different from the target masses. The concentrations shown below reflect the actual concentrations made for the stock solutions. I used a 50 mL volumetric flask to make the gold stock solution and a 100 mL volumetric flask to make the protein solution. In each case I used HPLC-grade water.

Stock solutions

  1. HAuCl4·H2O
    1. X mg
    2. MW = 339.79 g/mol
    3. Volume = 50 mL
    4. Y mM
  2. Lysozyme
    1. A mg
    2. MW = 14307 g/mol
    3. Volume = 100 mL
    4. B μM

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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