User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2014/08/29

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Objective

Completing film synthesis, starting new film synthesis, and making stock Malachite green solutions.

Description

  1. Completing film synthesis
    1. Place film in Sodium Sulfate Solution (see below for details) at 80C for 1 hour with gentle stirring
    2. Place film in 1% HCl Solution (see below for details) at 80C for 30 minutes with gentle stirring
    3. Place film in Sodium Bicarbonate Solution for 15 minutes at room temperature with gentle stirring
  2. Starting new film synthesis
    1. Use Wednesday's Procedure to make new 10% clay-polyvinyl alcohol film
      1. It was 10% by weight clay because we used 1g of PVA and 0.11g of clay
    2. I also want you to make a 5% clay film using the appropriate amount of clay per 1 g of PVA.
  3. Malachite Green Solutions
    1. We need to make the following solutions
      1. 25mL 0.20ppm Malachite Green
      2. 25mL 0.50ppm Malachite Green
      3. 25mL 1.1ppm Malachite Green
      4. 25mL 1.4ppm Malachite Green
      5. 25mL 1.7ppm Malachite Green
      6. 50mL 2.0ppm Malachite Green
      7. 50mL 8.0ppm Malachite Green
      8. 50mL 80.0ppm Malachite Green
      9. 50mL 200.0ppm Malachite Green
        1. Note: I suggest that the groups talk amongst themselves to determine who is going to make which samples so that we aren't making duplicate. Hint: Someone needs to make the first stock solution that all of the other solutions are made from. Also: ppm is a mass/mass concentration.
    2. Take a UV-Visible spectrum of the 0.2ppm to 8ppm standards.
    3. Make a standard curve to determine the molar absorptivity of Malachite Green

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


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