User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2014/03/11: Difference between revisions

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I made HbAuNP samples similar to [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/12/05 | this procedure]]. Also, there are two ways to think about the ratio of Au:Hb. Hb is a tetramer. So, the molar mass is either 16125 (for the monomer) or 64500 (for the tetramer). The ratios listed below are for the tetramer/monomer. The ratios as reported in the referenced notebook page are those for the monomer.
I made HbAuNP samples similar to [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/12/05 | this procedure]]. Also, there are two ways to think about the ratio of Au:Hb. Hb is a tetramer. So, the molar mass is either 16125 (for the monomer) or 64500 (for the tetramer). The ratios listed below are for the tetramer/monomer. The ratios as reported in the referenced notebook page are those for the monomer.
# Au:Hb Ratio = 30
# Au:Hb Ratio = 30/7.5
## 500uL gold
## 500uL gold
## 1766uL Hb
## 1766uL Hb
## 2734uL water
## 2734uL water
# Au:Hb Ratio = 50
# Au:Hb Ratio = 50/12.5
## 500uL gold
## 500uL gold
## 1059uL Hb
## 1059uL Hb
## 757uL water
## 757uL water
# Au:Hb Ratio = 70
# Au:Hb Ratio = 70/17.5
## 500uL gold
## 500uL gold
## 757uL Hb
## 757uL Hb
## 3743uL water
## 3743uL water
# Au:Catalase Ratio = 90
# Au:Catalase Ratio = 90/22.5
## 500uL gold
## 500uL gold
## 589uL Hb
## 589uL Hb
## 3911uL water
## 3911uL water
# Au:Catalase Ratio = 110
# Au:Catalase Ratio = 110/27.5
## 500uL gold
## 500uL gold
## 482uL Hb
## 482uL Hb
## 4018uL water
## 4018uL water
# Au:Catalase Ratio = 130
# Au:Catalase Ratio = 130/32.5
## 500uL gold
## 500uL gold
## 407uL Hb
## 407uL Hb
## 4093uL water
## 4093uL water
# Au:Catalase Ratio = 150
# Au:Catalase Ratio = 150/37.5
## 500uL gold
## 500uL gold
## 353uL Hb
## 353uL Hb
## 4147uL water
## 4147uL water
# Au:Catalase Ratio = 170
# Au:Catalase Ratio = 170/42.5
## 500uL gold
## 500uL gold
## 312uL Hb
## 312uL Hb
## 4188uL water
## 4188uL water
# Au:Catalase Ratio = 190
# Au:Catalase Ratio = 190/47.5
## 500uL gold
## 500uL gold
## 279uL Hb
## 279uL Hb
## 4221uL water
## 4221uL water
# Au:Catalase Ratio = 210
# Au:Catalase Ratio = 210/52.5
## 500uL gold
## 500uL gold
## 252uL Hb
## 252uL Hb

Revision as of 12:57, 19 March 2014

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Objective

Making new catalase and Hb AuNP samples, as described here for AA and UVVis analysis.

Description

I made the following solutions:

  1. HAuCl4
    1. 24.6mg
    2. 25.0 mL water
  2. Hemoglobin
    1. 15.2mg
    2. 10.0 mL water
  3. Catalase
    1. 40.8mg
    2. 10.0 mL water

I made HbAuNP samples similar to this procedure. Also, there are two ways to think about the ratio of Au:Hb. Hb is a tetramer. So, the molar mass is either 16125 (for the monomer) or 64500 (for the tetramer). The ratios listed below are for the tetramer/monomer. The ratios as reported in the referenced notebook page are those for the monomer.

  1. Au:Hb Ratio = 30/7.5
    1. 500uL gold
    2. 1766uL Hb
    3. 2734uL water
  2. Au:Hb Ratio = 50/12.5
    1. 500uL gold
    2. 1059uL Hb
    3. 757uL water
  3. Au:Hb Ratio = 70/17.5
    1. 500uL gold
    2. 757uL Hb
    3. 3743uL water
  4. Au:Catalase Ratio = 90/22.5
    1. 500uL gold
    2. 589uL Hb
    3. 3911uL water
  5. Au:Catalase Ratio = 110/27.5
    1. 500uL gold
    2. 482uL Hb
    3. 4018uL water
  6. Au:Catalase Ratio = 130/32.5
    1. 500uL gold
    2. 407uL Hb
    3. 4093uL water
  7. Au:Catalase Ratio = 150/37.5
    1. 500uL gold
    2. 353uL Hb
    3. 4147uL water
  8. Au:Catalase Ratio = 170/42.5
    1. 500uL gold
    2. 312uL Hb
    3. 4188uL water
  9. Au:Catalase Ratio = 190/47.5
    1. 500uL gold
    2. 279uL Hb
    3. 4221uL water
  10. Au:Catalase Ratio = 210/52.5
    1. 500uL gold
    2. 252uL Hb
    3. 4248uL water

In order to get a more detailed view of the Catalase trends I made the following solutions:

  1. Au:Catalase Ratio = 7
    1. 500uL gold
    2. 2556uL catalase
    3. 1944uL water
  2. Au:Catalase Ratio = 17
    1. 500uL gold
    2. 1096uL catalase
    3. 3404uL water
  3. Au:Catalase Ratio = 26
    1. 500uL gold
    2. 697uL catalase
    3. 3803uL water
  4. Au:Catalase Ratio = 36
    1. 500uL gold
    2. 511uL catalase
    3. 3989uL water
  5. Au:Catalase Ratio = 46
    1. 500uL gold
    2. 404uL catalase
    3. 4096uL water
  6. Au:Catalase Ratio = 55
    1. 500uL gold
    2. 333uL catalase
    3. 4167uL water
  7. Au:Catalase Ratio = 65
    1. 500uL gold
    2. 284uL catalase
    3. 4216uL water
  8. Au:Catalase Ratio = 74
    1. 500uL gold
    2. 247uL catalase
    3. 4253uL water
  9. Au:Catalase Ratio = 84
    1. 500uL gold
    2. 219uL catalase
    3. 4281uL water
  10. Au:Catalase Ratio = 93
    1. 500uL gold
    2. 197uL catalase
    3. 4303uL water

I also took UVVis of the gold, Hb, and catalase stock solutions.

For each, I measured the spectrum for a 10X dilution of each stock solution.

Data

Here is a picture of the HbAuNPs (left - 30, right - 210)

Here is a picture of the CatalaseAuNPs (left - 390, right - 30)

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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