User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2014/02/06

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(Description)
(Description)
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#10mM Mn(CH<sub>3</sub>COO)<sub>2</sub>
#10mM Mn(CH<sub>3</sub>COO)<sub>2</sub>
## FW = 173.02 g/mol
## FW = 173.02 g/mol
-
## V = 250mL
+
## V = 50mL
## mass to be measured = 0.0865g
## mass to be measured = 0.0865g
## actual mass =  
## actual mass =  

Revision as of 13:22, 6 February 2014

Biomaterials Design Lab Main project page
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Objective

To synthesize BSA-ZnmMnn-mSn quantum dots using the protocol described here. We do not have a subscription to this journal. So ask me for a copy of the paper if you want one.

Description

Solutions to be made

  1. BSA/NaBH4
    1. Dissolve 4.14g of BSA in 60mL of water (That's 69 mg/mL BSA)
    2. Add 0.4g NaBH4 (That's 6.7 mg/mL NaBH4)
    3. Stir until no more bubbles (H2) are generated.
      1. Note NaBH4 can be dangerous to work with see here and here.
      2. I will be doing all of the handling of this material until researchers add it to their reactions.
  2. 100mM Zn(CH3COO)2
    1. FW = 219.49 g/mol
    2. V = 50mL
    3. mass to be measured = 1.0975g
    4. actual mass =
    5. actual concentration =
  3. 10mM Mn(CH3COO)2
    1. FW = 173.02 g/mol
    2. V = 50mL
    3. mass to be measured = 0.0865g
    4. actual mass =
    5. actual concentration =
  4. 100mM Na2S (Note: paper uses 0.25M)
    1. FW = 240.18 g/mol
    2. V = 50mL
    3. mass to be measured = 1.201g
    4. actual mass =
    5. actual concentration =

Protocol

  1. Start preparing a 70C water bath
  2. Add a stir bar to a 100mL round bottom flask
  3. Add 1 mL of BSA/NaBH4 to the flask
  4. Add 2.25mL of Zn stock solution
    1. Note: Solution will form a white precipitate upon addition of Zn2+
  5. Add 2.5mL of Mn stock solution
  6. Adjust the volume to 19mL using 13.25mL of water
  7. Stir mixture
  8. Adjust pH of solution to 9 with 1M NaOH
    1. Note how much NaOH you ultimately use
    2. Adding NaOH will help dispel the precipitate
  9. Seal container with a rubber septum
  10. Bubble N2 through the sample for 20 minutes
    1. Use the protocol we previously developed
  11. Add 2.5mL of Na2S solution
  12. Put the reaction vessel in the water bath and stir for three hours
  13. Store reaction mixture at 4C until later use.

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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