User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2014/02/06: Difference between revisions
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==Description== | ==Description== | ||
# Add | <u>Solutions to be made</u> | ||
# BSA/NaBH<sub>4</sub> | |||
## Dissolve 4.14g of BSA in 60mL of water (That's 69 mg/mL BSA) | |||
## Add 0.4g NaBH<sub>4</sub> (That's 6.7 mg/mL NaBH<sub>4</sub>) | |||
## Stir until no more bubbles (H<sub>2</sub>) are generated. | |||
### Note NaBH<sub>4</sub> can be dangerous to work with see [http://en.wikipedia.org/wiki/Sodium_borohydride#Safety here] and [http://www.sciencelab.com/msds.php?msdsId=9924969 here]. | |||
### I will be doing all of the handling of this material until researchers add it to their reactions. | |||
#100mM Zn(CH<sub>3</sub>COO)<sub>2</sub> | |||
## FW = 219.49 g/mol | |||
## V = 50mL | |||
## mass to be measured = 1.0975g | |||
## actual mass = | |||
## actual concentration = | |||
#10mM Mn(CH<sub>3</sub>COO)<sub>2</sub> | |||
## FW = 173.02 g/mol | |||
## V = 250mL | |||
## mass to be measured = 0.0865g | |||
## actual mass = | |||
## actual concentration = | |||
#100mM Na<sub>2</sub>S (Note: paper uses 0.25M) | |||
## FW = 240.18 g/mol | |||
## V = 50mL | |||
## mass to be measured = 1.201g | |||
## actual mass = | |||
## actual concentration = | |||
<u>Protocol</u> | |||
# Start preparing a 70C water bath | |||
# Add a stir bar to a 100mL round bottom flask | |||
# Add 1 mL of BSA/NaBH<sub>4</sub> to the flask | |||
# Add 2.25mL of Zn stock solution | |||
## Note: Solution will form a white precipitate upon addition of Zn<sup>2+</sup> | |||
# Add 2.5mL of Mn stock solution | |||
# Stir mixture | |||
# Adjust pH of solution to 9 with 1M NaOH | |||
## Note how much NaOH you ultimately use | |||
## Adding NaOH will help dispel the precipitate | |||
# Seal container with a rubber septum | |||
# Bubble N<sub>2</sub> through the sample for 20 minutes | |||
## Use the protocol we previously [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2014/01/24 | developed]] | |||
# Add 2.5mL of Na<sub>2</sub>S solution | |||
# Put the reaction vessel in the water bath and stir for three hours | |||
# Store reaction mixture at 4C until later use. | |||
==Data== | ==Data== |
Revision as of 09:18, 6 February 2014
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ObjectiveTo synthesize BSA-ZnmMnn-mSn quantum dots using the protocol described here. We do not have a subscription to this journal. So ask me for a copy of the paper if you want one. DescriptionSolutions to be made
Protocol
Data
NotesThis area is for any observations or conclusions that you would like to note.
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