User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2014/02/06: Difference between revisions
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#### I began chilling this in the freezer as soon as I put the reaction in the 50C water bath. | #### I began chilling this in the freezer as soon as I put the reaction in the 50C water bath. | ||
### I stopped at this point because my QDs did not precipitate out of solution. Will need to contact author. I tried ethanol as well as acetone (as I had seen in other reports) | ### I stopped at this point because my QDs did not precipitate out of solution. Will need to contact author. I tried ethanol as well as acetone (as I had seen in other reports) | ||
### I placed the tubes in the freezer | |||
### After about 10 minutes I did notice some precipitate. I'm going to let them ppt longer (overnight and collect them tomorrow). | |||
### The solid is collected by centrifugation (5 minutes at 5000 rpm) | ### The solid is collected by centrifugation (5 minutes at 5000 rpm) | ||
### The solid is re-dissolved in a minimum amount of cold water | ### The solid is re-dissolved in a minimum amount of cold water |
Revision as of 11:35, 6 February 2014
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Objective
Description1. BSA-ZnmMnn-mSn quantum dot prep Solutions to be made
Protocol
2. cysteine-ZnmMnn-mSn quantum dot synthesis Note: Eleni, James, and Andrew ran this procedure yesterday. It took more than 60uL to bring the pH up to 11. While they were changing the pH, a clear precipitate formed in solution. Upon addition of 2 more mL of 1M NaOH, the precipitate dissolved back into solution, and the solution turned an orange'ish color. I also observed the presence of a black precipitate suspended in solution. When I run this today, I'm going to add NaOH drop wise until the original ppt goes away and then check the pH.
Data
NotesThis area is for any observations or conclusions that you would like to note.
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