User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2014/01/31: Difference between revisions

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## V = 250mL
## V = 250mL
## mass to be measured = 0.274g
## mass to be measured = 0.274g
## actual mass =  
## actual mass = 0.274g
## actual concentration =
## actual concentration = 5mM
#5mM Mn(CH<sub>3</sub>COO)<sub>2</sub>
#5mM Mn(CH<sub>3</sub>COO)<sub>2</sub>
## FW = 173.02 g/mol
## FW = 173.02 g/mol
## V = 250mL
## V = 250mL
## mass to be measured = 0.216g
## mass to be measured = 0.216g
## actual mass =  
## actual mass = 0.220 g (slightly pinkish hue)
## actual concentration =
## actual concentration = 5.1mM
#40mM NH<sub>4</sub>(CH<sub>3</sub>COO)<sub>2</sub>
#40mM NH<sub>4</sub>(CH<sub>3</sub>COO)<sub>2</sub>
## FW = 77.08 g/mol
## FW = 77.08 g/mol
## V = 250mL
## V = 250mL
## mass to be measured = 0.7708g
## mass to be measured = 0.7708g
## actual mass =  
## actual mass = 0.780g
## actual concentration =
## actual concentration = 40.5mM
#10mM NH<sub>4</sub>OH
#10mM NH<sub>4</sub>OH
## initial M = 14.5M
## initial M = 14.5M

Revision as of 09:46, 31 January 2014

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

Synthesize Mn-doped ZnS quantum dots using the synthesis described here

Description

Synthesis Protocol for Mn-doped ZnS nanocrystals with an Mn:Zn ratio of 1:7.5 and a final (Zn+Mn) concentration of 0.4mM

  1. Add 176μL of 5mM Zn(CH3COO)2 to a test tube
  2. Add 24μL of 5mM Mn((CH3COO)2) to the test tube
  3. Add 200μL of 40mM NH4CH3COO to the test tube
  4. Add 244μL of 0.55mg/mL protein to the test tube
  5. Add 250μL of 10mM NH4OH
    1. This should bring the pH up to 8.2. Check on this with a pH strip
  6. Bring the volume up to 2.5mL with water (Add 1606μL of water)
  7. Incubate at room temperature for 1 hour
  8. Add 200μL of 5mM Na2S
    1. When you add this add it drop wise (as best as you can) while vigorously shaking the test tube.
  9. Incubate at 37C for 5 days.

Data

Stock Solutions

All solutions are made in 250mL volumetric flasks.

  1. 5mM Zn(CH3COO)2
    1. FW = 219.49 g/mol
    2. V = 250mL
    3. mass to be measured = 0.274g
    4. actual mass = 0.274g
    5. actual concentration = 5mM
  2. 5mM Mn(CH3COO)2
    1. FW = 173.02 g/mol
    2. V = 250mL
    3. mass to be measured = 0.216g
    4. actual mass = 0.220 g (slightly pinkish hue)
    5. actual concentration = 5.1mM
  3. 40mM NH4(CH3COO)2
    1. FW = 77.08 g/mol
    2. V = 250mL
    3. mass to be measured = 0.7708g
    4. actual mass = 0.780g
    5. actual concentration = 40.5mM
  4. 10mM NH4OH
    1. initial M = 14.5M
    2. final V = 250mL
    3. volume to be diluted = 172μL
    4. actual concentration =

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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