User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/10/16: Difference between revisions
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==Objective== | ==Objective== | ||
We are going to test the activity of our HRP-NPs today for the catalytic conversion of | We are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol | ||
==Description== | ==Description== | ||
Add experimental record here. Include what, how, and why... | |||
==Data== | ==Data== | ||
<u>Stock Solution</u> | |||
# Buffer | |||
## 0.6140g Tris in 1L, pH set to 8 with HCl ---> 5.1mM | |||
# Luminol | |||
## Dissolve 12.9mg luminol in 300uL of DMSO | |||
## Add to 50mL buffer ---> 1.46mM | |||
# H2O2 | |||
## 312uL 30% H2O2 into 100mL buffer ---> Should be 45mM | |||
## Check absorption at 250 [http://www.bio.net/mm/methods/1995-January/023533.html source] (ε(250) = 16.69 M<sup>-1</sup>cm<sup>-1</sup>). A = 0.7392. [H2O2] = 44.29mM | |||
==Notes== | ==Notes== |
Revision as of 11:48, 22 October 2013
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ObjectiveWe are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol DescriptionAdd experimental record here. Include what, how, and why... DataStock Solution
NotesThis area is for any observations or conclusions that you would like to note.
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