User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/10/08: Difference between revisions
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==Description== | ==Description== | ||
# | # Make a 40μM solution of adenosine in buffer (50mM phosphate buffer, pH 7.4) | ||
# Go to Dr. Hartings lab for enzyme kinetics measurements. | |||
## Add 3mL of adenosine solution to the cuvette | |||
## Start your kinetics measurement | |||
### 1ms integration (on front panel) | |||
### 10 scan average (on front panel) | |||
### Save a scan once every 15 seconds (after clicking File>Save) | |||
### Stop saving scans after 10 minutes (after clicking File>Save) | |||
### Give the files a directory and a name | |||
### Click accept | |||
### Just before 1 minute add 30ul of 0.01u/mL ADA | |||
==Data== | ==Data== |
Revision as of 07:59, 8 October 2013
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ObjectiveTo observe and measure ADA turnover kinetics in the absence of an inhibitor. This work will be the basis of our comparison to ADA-AuNP turnover studies. Description
Data
NotesThis area is for any observations or conclusions that you would like to note.
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