User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/10/01: Difference between revisions
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==Data== | ==Data== | ||
<u>Stock Solution</u> | |||
# Buffer | |||
## 0.6175g Tris in 1L, pH set to 8 with HCl ---> 5.1mM | |||
# HRP | |||
## 1.7mg in 50mL buffer (MW ~ 44,000) ---> 0.77uM | |||
# Luminol | |||
## Dissolve 13.4mg luminol in 300uL of DMSO | |||
## Add to 50mL buffer ---> 1.51mM | |||
# H2O2 | |||
## 177uL 30% H2O2 into 50mL buffer ---> Should be 45mM | |||
## Check this concentration! The molar absorptivity of H2O2 at 240nm is 40,000 M<sup>-1</sup>cm<sup>-1</sup> | |||
==Notes== | ==Notes== |
Revision as of 08:52, 1 October 2013
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ObjectiveTo monitor the kinetics and yield of the horseradish peroxidase-catalyzed oxidation of luminol. These experiments will be compared to future experiments with HRP-functionalized nanoparticles. These experiments are also meant to introduce researchers to stopped-flow techniques and rapid data collection. DescriptionThree sets of measurements will be performed today.
DataStock Solution
NotesThis area is for any observations or conclusions that you would like to note.
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