User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/09/04: Difference between revisions
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==Notes== | ==Notes== | ||
I made a stock solution of BSA today. Here is the info: | |||
BSA solution 0.0104g BSA (MW = 66776g/mol) in 0.0100mL water → 15.6μM | |||
[[Category:Course]] | [[Category:Course]] |
Revision as of 09:00, 4 September 2013
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ObjectiveBecome familiar with the fluorescence spectrometer and with protein fluorescence. DescriptionFluorescence spectroscopy is another way to analyze molecular samples. InstructionsEach group will pick a different protein (bovine serum albumin, horseradish peroxidase, pepsin, adenosine deaminase, hemoglobin). You'll need to make 4 samples ranging from 10uM to 0.5uM. Remember, we don't have a lot of most of these proteins, so figure out how to make a stock solution using as little solid protein as possible. The fluorescence cuvette needs only 200uL, so you don't need to make more than that for each concentration that you're measuring.
NotesI made a stock solution of BSA today. Here is the info: BSA solution 0.0104g BSA (MW = 66776g/mol) in 0.0100mL water → 15.6μM
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