User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2012/09/12: Difference between revisions
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==Description== | ==Description== | ||
# Add | Following transformation protocol I normally use: | ||
#Place plastic culture tubes on ice for 15 minutes | |||
#Add cells to ice to thaw | |||
#Add 1uL of DNA to tubes | |||
#Place on ice for 1 minute | |||
#Place 30uL of cells over DNA | |||
#Incubate for 4 minutes | |||
#Heat shock for 80 seconds | |||
#Put cells on ice | |||
#Add 100uL of SOC | |||
#Shake at 37C for 1 hr | |||
#Plate on kanamycin LB plates | |||
==Data== | ==Data== |
Revision as of 08:52, 17 September 2012
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ObjectiveTransform BL21(DE3) cells with the DNA extracted yesterday. DescriptionFollowing transformation protocol I normally use:
Data
NotesThis area is for any observations or conclusions that you would like to note.
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