User:Mary Mendoza/Notebook/CHEM 581: Experimental Chemistry I/2014/10/31: Difference between revisions

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* Acetonitrile was tested without the use of the solvent miscibility chart. Upon checking the chart, the solubility in water of acetonitrile (%w/w) is 100.
* Acetonitrile was tested without the use of the solvent miscibility chart. Upon checking the chart, the solubility in water of acetonitrile (%w/w) is 100.


*  
* Hence, I selected the top 5 least immiscible organic solvent according to their solubility in water (%w/w) as shown in the picture.
 
[[Image:Solvents.png]]




Revision as of 09:50, 11 December 2014

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Continuation of Bead Synthesis Trials

  • The burette set-up utilized by Eleni, Melvin, and Becca's group will be used; details of their assembly can be found on Kalivas' entry for 10/29/2014.
  • The assembly was prepared as shown in the picture.

Description

  1. A column was set up with glutaraldehyde and hexane. Air is being bubbled through the column to mix the two liquids.
  2. PVA-Clay will be dropped down the column

Hexane Trial

  • The PVA-Clay formed beads within the hexane and floated down the column. They did not cross-link fast enough if at all and they dissolved into the glutaraldehyde.

Organic-Glutaraldehyde Solution

  • We attempted to add acetone to glutaraldehyde and then add hexane to get rid of the solvent layers. The solution was not hydrophobic enough to form beads however. When only a slight layer of hexane and stirring were applied, it yielded no good results.
  1. We have decided to abandon the beads and begin synthesis of ribbons
    1. We filled a column with acetone and 1.5mL of Glutaraldehyde
    2. we then dropped in the PVA-Clay

Trial of Several Organic Solvents

  • I observed that as soon as the organic phase, formed bead comes into contact with the aqueous phase of the glutaraldehyde, the bead disperses into the aqueous solution. I decided to conduct my own trials and have my group members continue the burette-ribbon experiments.
  • I concluded that the dilemma in the formation of beads is the immiscibility of the organic phase which forms the beads and the aqueous phase that cross-links the beads.
  • Therefore, I thought, I can resolve this issue by choosing several organic solvents slightly miscible with water but hydrophobic enough to form the beads.
  • In an attempt to keep the reaction mixture homogenized, motion is required. Thus, the reaction should be kept stirring on a stir plate. Also, stirring prevents the formed beads from clumping together."
  • I used the solvent miscibility table provided by Dr. Hartings taken from chemical company, phenomenex, as shown in the picture.

Acetonitrile Trial

  • A drop of the PVA-clay was added into 3 mL of acetonitrile contained in a 12x100 mm, 8 mL disposable test tube.
  • The PVA-clay did not form beads.
  • Acetonitrile was tested without the use of the solvent miscibility chart. Upon checking the chart, the solubility in water of acetonitrile (%w/w) is 100.
  • Hence, I selected the top 5 least immiscible organic solvent according to their solubility in water (%w/w) as shown in the picture.



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