User:Mary Mendoza/Notebook/CHEM 581: Experimental Chemistry I/2014/10/24: Difference between revisions
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* Once the column was ready, 10 mL BPA sample was poured into the column; solvent of the BPA was drained through the column. | * Once the column was ready, 10 mL BPA sample was poured into the column; solvent of the BPA was drained through the column. | ||
* When most of the sample solvent has been drained, 10 mL of water was added into the column. | * When most of the sample solvent has been drained, 10 mL of water was added into the column. | ||
* 2 fractions of the 10 mL water and 6 fractions of the 30 mL methanol were collected. Each fraction was dotted onto a thin layer chromatography (TLC) paper. | * 2 fractions of the 10 mL water and 6, 5 mL fractions of the 30 mL methanol were collected. Each fraction was dotted onto a thin layer chromatography (TLC) paper. | ||
* After dotting the TLC paper, there were no observed changes. Hence, Dr. Hartings dissolved FeCl<sub>3</sub> in methanol. FeCl<sub>3</sub> is expected to form a blue compound once added to phenolic compounds. However, no change was observed even with the addition of FeCl<sub>3</sub>. | * After dotting the TLC paper, there were no observed changes. Hence, Dr. Hartings dissolved FeCl<sub>3</sub> in methanol. FeCl<sub>3</sub> is expected to form a blue compound once added to phenolic compounds. However, no change was observed even with the addition of FeCl<sub>3</sub>. | ||
* The experiment was terminated. | * The experiment was terminated. |
Revision as of 20:29, 10 December 2014
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Column Chromatography for BPA Concentration
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