User:Mary Mendoza/Notebook/CHEM 581: Experimental Chemistry I/2014/10/24: Difference between revisions
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* The column used was a 30 cc plastic syringe. The first layer was filled with glass wool fiber and fine sand. The sand was ground by Dr. Hartings using mortar and pestle. | * The column used was a 30 cc plastic syringe. The first layer was filled with glass wool fiber and fine sand. The sand was ground by Dr. Hartings using mortar and pestle. | ||
* 9.9999 g of BDH Silica Gel 60 Angstroms High Purity 40-63 μm was mixed with approximately 30 mL of methanol. | * 9.9999 g of BDH Silica Gel 60 Angstroms High Purity 40-63 μm was mixed with approximately 30 mL of methanol. | ||
[[Image:IMG 3210.JPG|thumb|center|600x400px|BDH Silica Gel 60 Angstroms High Purity 40-63 μm]] | |||
* First, the column was washed with water. Afterwards, the silica-methanol mixture was slowly poured to leave the sand layer undisturbed. | * First, the column was washed with water. Afterwards, the silica-methanol mixture was slowly poured to leave the sand layer undisturbed. | ||
* After some of the solvent front, methanol, passed, the column was washed with water again. Air pressure was applied to compact the column. | * After some of the solvent front, methanol, passed, the column was washed with water again. Air pressure was applied to compact the column. |
Revision as of 20:27, 10 December 2014
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Column Chromatography for BPA Concentration
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