User:Mary Mendoza/Notebook/CHEM 581: Experimental Chemistry I/2014/10/01: Difference between revisions
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* Using a vernier caliper, the thickness of the PVA-clay film used for diffusion is 0.51 mm. | * Using a vernier caliper, the thickness of the PVA-clay film used for diffusion is 0.51 mm. | ||
* The diffusion assembly was prepared according to the picture below, containing 10 mL of 20 ppm Malachite green on the left chamber and 10 mL of distilled, deionized water: | * The diffusion assembly was prepared according to the picture below, containing 10 mL of 20 ppm Malachite green on the left chamber and 10 mL of distilled, deionized water: | ||
[[Image:10 1 MG Diffusion1 916AM.JPG|thumb|300x300px| | [[Image:10 1 MG Diffusion1 916AM.JPG|thumb|300x300px|left|Status at 9:16 AM]] | ||
[[Image:10 1 MG Diffusion2 916AM.JPG|thumb|300x300px| | [[Image:10 1 MG Diffusion2 916AM.JPG|thumb|300x300px|right|Status at 9:16 AM]] | ||
[[Image:10 1 MG Diffusion1 738PM.JPG|thumb|300x300px| | [[Image:10 1 MG Diffusion1 738PM.JPG|thumb|300x300px|left|Status at 7:38 PM]] | ||
* 10 uL of the 20 ppm Malachite green stock was diluted into 200 uL of DI water. This mixture was transferred into a 200 uL quartz, fluorescence cuvette; the absorbance was measured as the zero time for the diffusion curve. The absorbance of 200 uL of DI water was directly measured as the zero mark for the diffusion curve. | * 10 uL of the 20 ppm Malachite green stock was diluted into 200 uL of DI water. This mixture was transferred into a 200 uL quartz, fluorescence cuvette; the absorbance was measured as the zero time for the diffusion curve. The absorbance of 200 uL of DI water was directly measured as the zero mark for the diffusion curve. |
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Malachite Green Diffusion through PVA-clay film
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