User:Mary Mendoza/Notebook/CHEM 581: Experimental Chemistry I/2014/09/17: Difference between revisions
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==UV-Vis of MG-PVA samples== | ==UV-Vis of MG-PVA samples== | ||
[[Image:9 17 MG PVA Abs.png|left|thumb|Table 1. Absorbance of Polyvinyl Alcohol Films in Malachite Green]] | |||
* Using quartz cuvettes, absorbance of 2 mL malachite green samples were collected. | * Using quartz cuvettes, absorbance of 2 mL malachite green samples were collected. | ||
* 80 and 200 ppm malachite green samples were diluted to 100x and 50x, respectively. | * 80 and 200 ppm malachite green samples were diluted to 100x and 50x, respectively. | ||
* The absorbance of each sample are listed on table 1. | |||
[[Image:9 17 MG PVA UV vis.png|center|thumb|800x458px|Absorbance Spectra of PVA films in Malachite Green]] | |||
==Centrifuge of Ionic Liquid Exchange== | ==Centrifuge of Ionic Liquid Exchange Clay== | ||
* In the original [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2014/09/10|protocol]], the instruction called for vacuum filtration of our mixture, tributylhexadecylphosphonium bromide, through a 0.2μm nylon fiber. | |||
* A consensual decision was made that placing the mixtures in centrifuge would provide better results of separation. | |||
* The sample weighed the amount of 17.69 g. Addition of water was made to balance the viscous mixture, (1-hexadecyl)triphenylphosphonium bromide), from Eleni's group. The final weight was 27.42 g. | |||
* The mixture was centrifuged for 30 minutes at 4000 rpm, 4°C. The centrifuge used was the make of Thermo Scientific Sorvall RC 6+ Centrifufge. | |||
* The rotor chosen to perform the centrifuge was SH-3000 4700 max. | |||
* At 10:53 AM, samples were observed to have three distinct layers; top and bottom layer were clay, and the middle was composed of water/supernatant. | |||
* The supernatant was collected and transferred into a 50 mL falcon tube. This was stored if additional analysis was needed in the future. | |||
* Two sets of washes are required for the exchanged clay. Each wash comprised of washing the clay with water to remove adsorbed NaCl and ethanol to remove remaining surfactants. | |||
* After the washes were completed by Dr. Hartings, the mixture was stored in an oven to evaporate excess water. | |||
==Powder X-RAY Diffraction of MG-PVA-clay samples== | ==Powder X-RAY Diffraction of MG-PVA-clay samples== | ||
* Eleni Kalivas executed the introduction of the usage of the powder X-RAY diffraction instrument. The make of the instrument is by Rigaku MiniFlex II. | |||
* The parameters set for the data collection made via the Standard Measurement interface are as follows: | |||
** 2° start angle | |||
** 40° stop angle | |||
** 0.5 inch sampling width | |||
** 1°/minute scanning speed | |||
*** The original [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2014/09/03|protocol]] stated a 1°/second scanning speed | |||
* Our group provided the blank PVA film which was placed in position 4. Other groups' PVA films were placed in the following order: | |||
** Position 1: Dr. Hartings' solid, sodium montmorillonite | |||
** Position 2: 8 ppm MG from Madeleine and Alicia | |||
** Position 3: group's 2 ppm MG | |||
** Position 5: 80 ppm MG from Melvin, Eleni, and Becca | |||
** Position 6: 200 ppm MG from Andrew, Michael, and Tami | |||
==Completion of Exfoliated Clay Film Synthesis== | ==Completion of Exfoliated Clay Film Synthesis== | ||
* This was performed by our group member, Jacob Esenther, following the [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2014/08/29|Completion of Film Synthesis protocol]] from August 29, 2014. | |||
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Revision as of 23:59, 29 September 2014
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Collection of DSC data
UV-Vis of MG-PVA samples
Centrifuge of Ionic Liquid Exchange Clay
Powder X-RAY Diffraction of MG-PVA-clay samples
Completion of Exfoliated Clay Film Synthesis
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