User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/27

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==Kinetics of ADA==
==Kinetics of ADA==
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* The activity of ADA was measured by monitoring the absorbance of inosine.  
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* The activity of ADA was measured by monitoring the absorbance of inosine over time.
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[[Image:AbsoofIIno.png]]
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* The trends among runs 1, 2, and 3 seem consistent. The increase of the concentration of the enzyme with each addition increases the rate of production of inosine with respect to time.
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NOTE: The remaining period was used to edit formatting for Class Literature Survey
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|''Volume of ADA (μL)''
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| align="center" style="background:#f0f0f0;"|''Concentration of ADA (μM)''
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| align="center" style="background:#f0f0f0;"|''Volume of phosphate buffer (mL)''
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| align="center" style="background:#f0f0f0;"|''Concentration of phosphate buffer (M)''
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| align="center" style="background:#f0f0f0;"|''Volume of adenosine (μL)''
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| align="center" style="background:#f0f0f0;"|''Concentration of adenosine (mM)''
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|-
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| 15||0.21||2.95||0.0492||50||0.0512
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|-
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| 25||0.35||2.95||0.0492||50||0.0512
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|-
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| 40||0.56||2.95||0.0492||50||0.0512
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|-
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| 50||0.70||2.95||0.0492||50||0.0512
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|-
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Dialysis Results

  • Dialysis tubes were prepared for adenosine deaminase (ADA) fractions 2 and 8 on 11/14/12. The ADA were removed from the dialysis tubes.
  • ADA fraction 2 appeared clear and colorless with no signs of precipitation.
  • ADA fraction 8 precipitated out of solution.

Kinetics of ADA

  • The activity of ADA was measured by monitoring the absorbance of inosine over time.

Image:AbsoofIIno.png

  • The trends among runs 1, 2, and 3 seem consistent. The increase of the concentration of the enzyme with each addition increases the rate of production of inosine with respect to time.


NOTE: The remaining period was used to edit formatting for Class Literature Survey


Volume of ADA (μL) Concentration of ADA (μM) Volume of phosphate buffer (mL) Concentration of phosphate buffer (M) Volume of adenosine (μL) Concentration of adenosine (mM)
150.212.950.0492500.0512
250.352.950.0492500.0512
400.562.950.0492500.0512
500.702.950.0492500.0512


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