User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/27

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Revision as of 18:47, 9 December 2012

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Dialysis tubes were prepared for adenosine deaminase (ADA) fractions 2 and 8 on 11/14/12. The ADA were removed from the dialysis tubes.
ADA fraction 2 appeared clear and colorless with no signs of precipitation.
ADA fraction 8 precipitated out of solution.

The activity of ADA was measured by monitoring the absorbance of inosine over time.

The trends among runs 1, 2, and 3 seem consistent. The increase of the concentration of the enzyme with each addition increases the rate of production of inosine with respect to time.

Volume of ADA (μL)	Concentration of ADA (μM)	Volume of phosphate buffer (mL)	Concentration of phosphate buffer (M)	Volume of adenosine (μL)	Concentration of adenosine (mM)
15	0.21	2.95	0.0492	50	0.0512
25	0.35	2.95	0.0492	50	0.0512
40	0.56	2.95	0.0492	50	0.0512
50	0.70	2.95	0.0492	50	0.0512

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 [[Image:AbsoofIIno.png]]
+* The trends among runs 1, 2, and 3 seem consistent. The increase of the concentration of the enzyme with each addition increases the rate of production of inosine with respect to time.
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