User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/27: Difference between revisions

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{| {{table}}
| align="center" style="background:#f0f0f0;"|''Volume of ADA (μL)''
| align="center" style="background:#f0f0f0;"|''Concentration of ADA (μM)''
| align="center" style="background:#f0f0f0;"|''Volume of phosphate buffer (mL)''
| align="center" style="background:#f0f0f0;"|''Concentration of phosphate buffer (M)''
| align="center" style="background:#f0f0f0;"|''Volume of adenosine (μL)''
| align="center" style="background:#f0f0f0;"|''Concentration of adenosine (mM)''
|-
| 15||0.21||2.95||0.0492||50||0.0512
|-
| 25||0.35||2.95||0.0492||50||0.0512
|-
| 40||0.56||2.95||0.0492||50||0.0512
|-
| 50||0.70||2.95||0.0492||50||0.0512
|-


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Revision as of 13:45, 9 December 2012

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Dialysis Results

  • Dialysis tubes were prepared for adenosine deaminase (ADA) fractions 2 and 8 on 11/14/12. The ADA were removed from the dialysis tubes.
  • ADA fraction 2 appeared clear and colorless with no signs of precipitation.
  • ADA fraction 8 precipitated out of solution.

Kinetics of ADA

  • The activity of ADA was measured by monitoring the absorbance of inosine over time.


Volume of ADA (μL) Concentration of ADA (μM) Volume of phosphate buffer (mL) Concentration of phosphate buffer (M) Volume of adenosine (μL) Concentration of adenosine (mM)
15 0.21 2.95 0.0492 50 0.0512
25 0.35 2.95 0.0492 50 0.0512
40 0.56 2.95 0.0492 50 0.0512
50 0.70 2.95 0.0492 50 0.0512


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