User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/14: Difference between revisions
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==UV-vis of Adenosine and Inosine== | ==UV-vis of Adenosine and Inosine== | ||
* Due to the inconsistent behavior observed from the data above, it was decided to remeasure the absorbance of adenosine and inosine in the UV-visible spectra. The program was returned to the Spectrum setting. The measurement was adjusted to 400-200 nm. | |||
[[Image:AdenoIno2.png]] | |||
* The absorbance values are listed below in the table. The calculation for the molar absorptivities of adenosine and inosine are shown below. | |||
For adenosine | |||
* At 235 <math>\frac{\epsilon = .099}{.0000307 M}</math> = 3224.75 | |||
* At 265 <math>\frac{\epsilon = .24}{.0000307 M}</math> = 7817.59 | |||
For inosine | |||
* At 235 <math>\frac{\epsilon = .754}{.0001 M}</math> = 7540 | |||
* At 265 <math>\frac{\epsilon = .553}{.0001 M}</math> = 5530 | |||
* Running the UV-vis scan for each reagent showed that the increasing trend observed on the ADA kinetic assays was inosine not adenosine. This was verified by recalculating the molar absorptivities. The molar absortivity of inosine at 235 is greater than adenosine. This explains the increase over time since as ADA catalyzes the formation of inosine from adenosine; the catalysis increases the concentration of inosine so as its absorbance. | |||
* The kinetic assay with its corrected information will be continued a week after break. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''' | |||
| align="center" style="background:#f0f0f0;"|''adenosine'' | |||
| align="center" style="background:#f0f0f0;"|''inosine'' | |||
|- | |||
| A235||0.099||0.754 | |||
|- | |||
| Molar Absorptivity 235||3224.75||7540 | |||
|- | |||
| A265||0.24||0.553 | |||
|- | |||
|Molar Absorptivity 265||7817.59||5530 | |||
|- | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | |||
|} | |||
__NOTOC__ | |||
==Dialysis of ADA== | |||
* Fractions 2 and 8 of ADA were transferred to a dialysis tube and submerged in water for one week. | |||
Revision as of 13:15, 11 December 2012
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ADA Kinetic Assay Runs
UV-vis of Adenosine and Inosine
For adenosine
For inosine
Dialysis of ADA
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