User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/14: Difference between revisions

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| Volume||33 μL||27 μL||450 μL||88 μL||902 μL
| Volume||33 μL||27 μL||450 μL||88 μL||902 μL
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==UV-vis of Adenosine and Inosine==
==UV-vis of Adenosine and Inosine==

Revision as of 11:50, 9 December 2012

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ADA Kinetic Assay Runs

  • During the previous lab entry, the molar absorptivities of adenosine and inosine were calculated. Adenosine was found to have a higher molar absorptivity at 235.
  • It was concluded from the previous period that the absorption of adenosine should decrease over time. A kinetic assay scan of the reaction containing 2.7 mL of sodium phosphate buffer, 300 μL of adenosine, and 15 μL of ADA was taken.
  • The program was set to Kinetics with the wavelength at 235 for a collection span of 600s.


  • In the scan, it can be observed that the absorption of adenosine is not decreasing over time. Instead, the absorption is increasing. Also, the signal for the absorption of adenosine is over 1.
  • It was decided that the next measurements, the concentration of adenosine should be decreased by decreasing the volume in th cuvette. This is to ensure that the absorbance is below 1.



' HRP 4-iodophenol luminol H2O2 H2O
Molarity of stock solutions 2.3 μM 18 mM 10 mM 1.7 mM neutral
Volume 33 μL 27 μL 450 μL 88 μL 902 μL


UV-vis of Adenosine and Inosine


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