User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/14

ADA Kinetic Assay Runs

• During the previous lab entry, the molar absorptivities of adenosine and inosine were calculated. Adenosine was found to have a higher molar absorptivity at 235.
• It was concluded from the previous period that the absorption of adenosine should decrease over time. A UV-vis scan of a reaction containing 2.7 mL of sodium phosphate buffer, 300 μL of adenosine, and 15 μL of ADA was taken.