User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/14

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Revision as of 14:21, 9 December 2012

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During the previous lab entry, the molar absorptivities of adenosine and inosine were calculated. Adenosine was found to have a higher molar absorptivity at 235.
It was concluded from the previous period that the absorption of adenosine should decrease over time. A UV-vis scan of a reaction containing 2.7 mL of sodium phosphate buffer, 300 μL of adenosine, and 15 μL of ADA was taken.

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 ==ADA Kinetic Assay Runs==
 * During the previous lab entry, the molar absorptivities of adenosine and inosine were calculated. Adenosine was found to have a higher molar absorptivity at 235.
-* It was concluded from the previous period that the absorption of adenosine should decrease over time.
+* It was concluded from the previous period that the absorption of adenosine should decrease over time. A UV-vis scan of a reaction containing 2.7 mL of sodium phosphate buffer, 300 μL of adenosine, and 15 μL of ADA was taken.
+[[Image:Adeno300.png]]