User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/14: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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[[Image:Adaruns1114.png]] | [[Image:Adaruns1114.png]] | ||
* As seen above, decreasing the concentration of adenosine maintained an absorbance below 1. | * As seen above, decreasing the concentration of adenosine maintained an absorbance below 1. However, the absorbance is still increasing. The addition of 50 μL ADA intensified the increase of absorbance in comparison to the addition of 15 μL ADA. | ||
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==UV-vis of Adenosine and Inosine== | ==UV-vis of Adenosine and Inosine== | ||
* Due to the inconsistent behavior observed from the data above, it was decided to remeasure the absorbance of adenosine and inosine in the UV-visible spectra. The program was returned to the Spectrum setting. The measurement was adjusted to 400-200 nm. | |||
[[Image:AdenoIno2.png]] | |||
* The absorbance values are listed below in the table. The calculation for the molar absorptivities of adenosine and inosine are shown below. | |||
For adenosine | |||
* At 235 <math>\frac{\epsilon = .099}{.0000307 M}</math> = 3224.75 | |||
* At 265 <math>\frac{\epsilon = .24}{.0000307 M}</math> = 7817.59 | |||
For inosine | |||
* At 235 <math>\frac{\epsilon = .754}{.0001 M}</math> = 7540 | |||
* At 265 <math>\frac{\epsilon = .553}{.0001 M}</math> = 5530 | |||
* Running the UV-vis scan for each reagent showed that the increasing trend observed on the ADA kinetic assays was inosine not adenosine. This was verified by recalculating the molar absorptivities. The molar absortivity of inosine at 235 is greater than adenosine. This explains the increase over time since as ADA catalyzes the formation of inosine from adenosine; the catalysis increases the concentration of inosine so as its absorbance. | |||
* The kinetic assay with its corrected information will be continued a week after break. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''' | |||
| align="center" style="background:#f0f0f0;"|''adenosine'' | |||
| align="center" style="background:#f0f0f0;"|''inosine'' | |||
|- | |||
| A235||0.099||0.754 | |||
|- | |||
| Molar Absorptivity 235||3224.75||7540 | |||
|- | |||
| A265||0.24||0.553 | |||
|- | |||
|Molar Absorptivity 265||7817.59||5530 | |||
|- | |||
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|} | |||
__NOTOC__ | |||
==Dialysis of ADA== | |||
* Fractions 2 and 8 of ADA were transferred to a dialysis tube and submerged in water for one week. | |||
Latest revision as of 22:15, 26 September 2017
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ADA Kinetic Assay Runs
UV-vis of Adenosine and Inosine
For adenosine
For inosine
Dialysis of ADA
|