User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/13: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Mary Mendoza (talk | contribs) |
Mary Mendoza (talk | contribs) |
||
Line 87: | Line 87: | ||
For adenosine | For adenosine | ||
*At 235 <math>\frac{\epsilon = .57}{.0000307 M}</math> = 18566.78 | * At 235 <math>\frac{\epsilon = .57}{.0000307 M}</math> = 18566.78 | ||
* At 265 <math>\frac{\epsilon = .4}{.0000307 M}</math> = 13029.32 | * At 265 <math>\frac{\epsilon = .4}{.0000307 M}</math> = 13029.32 | ||
For inosine | |||
* At 235 <math>\frac{\epsilon = .14}{.0001 M}</math> = 1400 | |||
* At 265 <math>\frac{\epsilon = .6}{.0001 M}</math> = 6000 | |||
Revision as of 09:05, 9 December 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||
ADA Kinetic Assay Preparations
V1 = 0.08934 mL = 89.3 μL in 5 mL of buffer
UV-visible scans of Reagents
M1V1 = M2V2 (3 mM)(10 μL) = M2 (1000 μL) M2 = .0307 mM
3 μL of ADA × 65 μM = M2 (1000 μL) M2 = .195 μM of ADA
M2 = .0975 μM = 97.5 nM
Second dilution M = 48.8 nM Final Concentration = 24.38 nM
Beer's Law
|