User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/13
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(Difference between revisions)
(→Beer's Law) 
(→Beer's Law) 

Line 71:  Line 71:  
<math>\frac{\epsilon = A}{bc}</math>  <math>\frac{\epsilon = A}{bc}</math>  
  
* The calculation for the molar absorptivities of adenosine and inosine are shown below.  * The calculation for the molar absorptivities of adenosine and inosine are shown below.  
Line 81:  Line 80:  
* At 265 <math>\frac{\epsilon = .4}{.0000307 M}</math> = 13029.32  * At 265 <math>\frac{\epsilon = .4}{.0000307 M}</math> = 13029.32  
  
For inosine  For inosine  
  
* At 235 <math>\frac{\epsilon = .14}{.0001 M}</math> = 1400  * At 235 <math>\frac{\epsilon = .14}{.0001 M}</math> = 1400 
Revision as of 11:22, 9 December 2012
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ADA Kinetic Assay Preparations
V_{1} = 0.08934 mL = 89.3 μL in 5 mL of buffer
UVvisible scans of Reagents
M_{1}V_{1} = M_{2}V_{2} (3 mM)(10 μL) = M_{2} (1000 μL) M_{2} = .0307 mM
3 μL of ADA × 65 μM = M_{2} (1000 μL) M_{2} = .195 μM of ADA
M_{2} = .0975 μM = 97.5 nM
Second dilution M = 48.8 nM Final Concentration = 24.38 nM
Beer's Law
For adenosine
For inosine
