User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/13: Difference between revisions
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* At 265 <math>\frac{\epsilon = .6}{.0001 M}</math> = 6000 | * At 265 <math>\frac{\epsilon = .6}{.0001 M}</math> = 6000 | ||
* Evaluating the molar absorptivities for each wavelength, the results indicate that there is a greater difference of molar absorptivities between adenosine and inosine at wavelength 235. The molar absorptivity for adenosine was observed to be higher than inosine. | * Evaluating the molar absorptivities for each wavelength, the results indicate that there is a greater difference of molar absorptivities between adenosine and inosine at wavelength 235. The molar absorptivity for adenosine was observed to be higher than inosine. | ||
* We can hypothesized that before running the UV-Vis scans for the kinetic assay of ADA with adenosine, adenosine will produce a more pronounced absorption and signal. Therefore, adenosine should be monitored at 235. | * We can hypothesized that before running the UV-Vis scans for the kinetic assay of ADA with adenosine, adenosine will produce a more pronounced absorption and signal. Therefore, adenosine should be monitored at 235. | ||
Revision as of 09:21, 9 December 2012
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ADA Kinetic Assay Preparations
V1 = 0.08934 mL = 89.3 μL in 5 mL of buffer
UV-visible scans of Reagents
M1V1 = M2V2 (3 mM)(10 μL) = M2 (1000 μL) M2 = .0307 mM
3 μL of ADA × 65 μM = M2 (1000 μL) M2 = .195 μM of ADA
M2 = .0975 μM = 97.5 nM
Second dilution M = 48.8 nM Final Concentration = 24.38 nM
Beer's Law
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