User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/24: Difference between revisions
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* During the previous lab period of [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/23|10/23/12]], the Au/lysozyme solutions were incubated at 50°C. The solutions were observed to this date to exhibit no fibers. | * During the previous lab period of [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/23|10/23/12]], the Au/lysozyme solutions were incubated at 50°C. The solutions were observed to this date to exhibit no fibers. | ||
* Mole ratio 100 exhibited a slight purple tint. A UV-Vis scan was taken for this mole ratio. The graph of values are embedded below. | * Mole ratio 100 exhibited a slight purple tint. A UV-Vis scan was taken for this mole ratio. The graph of values are embedded below. | ||
[[Image:Lyso100.png]] | [[Image:Lyso100.png]] | ||
* The low temperature might have not denatured the lysozyme. Therefore, lysozyme could have not efficiently reduced Au (III) ito Au (0). The solution was re-incubated at a higher temperature, 85°C for 4h. | |||
* As advised by Dr. Miller, the solutions were checked during the incubation period. There were observable aggregation as seen below. The photo below is for the mole ratio 170. | |||
[[Image:2012-10-24 15.21.48.jpg]] | |||
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Revision as of 12:28, 27 October 2012
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Transformation of BL21 cellsThe following procedures were conducted near the proximity of the Bunsen burner in accordance with aseptic techniques. The procedure is based from the laboratory Transformation protocol.
UV-Vis of Au/lysozyme solution
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