User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/24: Difference between revisions
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* Delivered 250 μL of the LB and SOC media using an automatic delivery pipet into the labeled tubes. | * Delivered 250 μL of the LB and SOC media using an automatic delivery pipet into the labeled tubes. | ||
* The tubes containing the media were centrifuge for 1 h. at 37°C by Dr. Miller. | * The tubes containing the media were centrifuge for 1 h. at 37°C by Dr. Miller. | ||
* | * During the latter of the lab period, 2 plates were inoculated for each cell grown in LB and SOC media. | ||
* The first plate was inoculated with 50 μL of the BL21 cell grown in LB; the second plate was inoculate with a greater volume of cells with 200 μL. | |||
* Dhea Patel plated the BL21 cells grown in SOC media. | |||
Revision as of 12:12, 27 October 2012
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Transformation of BL21 cellsThe following procedures were conducted near the proximity of the Bunsen burner in accordance with aseptic techniques. The procedure is based from the laboratory Transformation protocol.
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