User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/24: Difference between revisions
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* After the 30 sec. mark, the tubes were submerged in ice. This rapid change in temperatures is a method of heat shock. This is an adequate of method of allowing the cells to incorporate the mutated plasmid into their intracellular matrix. | * After the 30 sec. mark, the tubes were submerged in ice. This rapid change in temperatures is a method of heat shock. This is an adequate of method of allowing the cells to incorporate the mutated plasmid into their intracellular matrix. | ||
* Delivered 250 μL of the LB and SOC media using an automatic delivery pipet into the labeled tubes. | * Delivered 250 μL of the LB and SOC media using an automatic delivery pipet into the labeled tubes. | ||
* The tubes containing the media were | * The tubes containing the media were placed on a shaker for 1 h. at 37°C by Dr. Miller. | ||
* During the latter of the lab period, 2 plates were inoculated for each cell grown in LB and SOC media. | * During the latter of the lab period, 2 plates were inoculated for each cell grown in LB and SOC media. | ||
* The first plate was inoculated with 50 μL of the BL21 cell grown in LB; the second plate was inoculate with a greater volume of cells with 200 μL. | * The first plate was inoculated with 50 μL of the BL21 cell grown in LB; the second plate was inoculate with a greater volume of cells with 200 μL. | ||
* Dhea Patel plated the BL21 cells grown in SOC media. | * Dhea Patel plated the BL21 cells grown in SOC media. | ||
==UV-Vis of Au/lysozyme solution== | ==UV-Vis of Au/lysozyme solution== |
Revision as of 13:39, 30 November 2012
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Transformation of BL21 cellsThe following procedures were conducted near the proximity of the Bunsen burner in accordance with aseptic techniques. The procedure is based from the laboratory Transformation protocol.
UV-Vis of Au/lysozyme solution
UV-Vis scans of Au/BSA
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