User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/23: Difference between revisions

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==AAS of Au/BSA solution of 10/17/12==
==AAS of Au/BSA solution of 10/17/12==
 
* The Au/BSA solutions were taken out from the oven. Mole ratios 60, 80, and 120 were the only solutions that exhibited a consistent, clear purple color with no fibers. This was indicative that all gold nanoparticles were in solution and none have nucleated to BSA.
* Mole ratios 100 and others produced fibers, having solutions clear and colorless. The liquid was taken from each solution and transferred to falcon tubes; leaving some liquid suspending the fibers in the original tube. As a result, they were irregular amounts on each tube.
* The irregular amounts created difficulty for centrifugation. Hours was spent in balancing the liquid for each pair of tubes.
* Time went by and the fibers formed pellets on the bottom of the tubes.




Revision as of 11:39, 27 October 2012

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ADA preparation

  • All ADA procedures for this lab period was conducted by Puja Mody.

Au/lysozyme solutions

  • The calculations were supposed to be posted on the notebook of Michael Nagle. This section will be updated as soon as additional information on Nagle would be added.

AAS of Au/BSA solution of 10/17/12

  • The Au/BSA solutions were taken out from the oven. Mole ratios 60, 80, and 120 were the only solutions that exhibited a consistent, clear purple color with no fibers. This was indicative that all gold nanoparticles were in solution and none have nucleated to BSA.
  • Mole ratios 100 and others produced fibers, having solutions clear and colorless. The liquid was taken from each solution and transferred to falcon tubes; leaving some liquid suspending the fibers in the original tube. As a result, they were irregular amounts on each tube.
  • The irregular amounts created difficulty for centrifugation. Hours was spent in balancing the liquid for each pair of tubes.
  • Time went by and the fibers formed pellets on the bottom of the tubes.



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