User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/23: Difference between revisions

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==Entry title==
==ADA preparation==
* Insert content here...
* All ADA procedures for this lab period was conducted by [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/23|Puja Mody]].  
 
==Au/lysozyme solutions==
* The calculations were supposed to be posted on the notebook of [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/23|Michael Nagle]]. This section will be updated as soon as additional information on Nagle would be added.
 
 
* When the Au/lysozyme solutions were made, Nagle placed the rack in the Thermo Scientific incubator at 50°C for 4h.
 
==AAS of Au/BSA solution of 10/17/12==
* The Au/BSA solutions were taken out from the oven. Mole ratios 60, 80, and 120 were the only solutions that exhibited a consistent, clear purple color with no fibers. This was indicative that all gold nanoparticles were in solution and none have nucleated to BSA.
* Mole ratios 100 and others produced fibers, having solutions clear and colorless. The liquid was taken from each solution and transferred to falcon tubes; leaving some liquid suspending the fibers in the original tube. As a result, they were irregular amounts on each tube.
* The irregular amounts created difficulty for centrifugation. Hours was spent in balancing the liquid for each pair of tubes.
* Time went by and the fibers formed pellets on the bottom of the tubes. The formation of pellets was acceptable to allow to take AAS of the solutions.
* The program for the Shimadzu AA-6200 Atomic Absorption Flame Emission was set on autozero. The flame was ignited exhibiting a blue-green color.
* The blank used for the experiment was water.
* The standards were run followed by an interval of sample-blank measurements. The calibration curve can be seen on the data section of [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/23|Puja Mody's notebook.]]
 




Revision as of 11:50, 27 October 2012

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ADA preparation

  • All ADA procedures for this lab period was conducted by Puja Mody.

Au/lysozyme solutions

  • The calculations were supposed to be posted on the notebook of Michael Nagle. This section will be updated as soon as additional information on Nagle would be added.


  • When the Au/lysozyme solutions were made, Nagle placed the rack in the Thermo Scientific incubator at 50°C for 4h.

AAS of Au/BSA solution of 10/17/12

  • The Au/BSA solutions were taken out from the oven. Mole ratios 60, 80, and 120 were the only solutions that exhibited a consistent, clear purple color with no fibers. This was indicative that all gold nanoparticles were in solution and none have nucleated to BSA.
  • Mole ratios 100 and others produced fibers, having solutions clear and colorless. The liquid was taken from each solution and transferred to falcon tubes; leaving some liquid suspending the fibers in the original tube. As a result, they were irregular amounts on each tube.
  • The irregular amounts created difficulty for centrifugation. Hours was spent in balancing the liquid for each pair of tubes.
  • Time went by and the fibers formed pellets on the bottom of the tubes. The formation of pellets was acceptable to allow to take AAS of the solutions.
  • The program for the Shimadzu AA-6200 Atomic Absorption Flame Emission was set on autozero. The flame was ignited exhibiting a blue-green color.
  • The blank used for the experiment was water.
  • The standards were run followed by an interval of sample-blank measurements. The calibration curve can be seen on the data section of Puja Mody's notebook.



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