User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17 - Revision history

Mary Mendoza: /* Preparation of Gold and Lysozyme stocks */

2012-12-07T14:18:02Z

Preparation of Gold and Lysozyme stocks

←Older revision		Revision as of 14:18, 7 December 2012
Line 26:		Line 26:

	==Preparation of Gold and Lysozyme stocks==		==Preparation of Gold and Lysozyme stocks==
-	* Michael Nagle prepared a new set of Au/BSA solutions. ~~However~~, ~~calculations made are not available in his notebook~~. Nagle finished the preparation of the set and placed the rack at 85°C for 4h. at the Thermo Scientific incubator.	+	* Michael Nagle prepared a new set of Au/BSA solutions. The stock concentration of BSA was 1.49 x 10<sup>-5</sup> mM/L and the stock concentration for Au was 8.24 mM. The mole ratios were kept the same as 60, 80, 100, 120, 128, 130, 132, 133, 134, 136, 138, 140, 160, and 170. Nagle finished the preparation of the set and placed the rack at 85°C for 4h. at the Thermo Scientific incubator.
	* Weighed out .014 g of monohydrate chloroauric acid and dissolved into 5 mL of water. The molarity of gold was calculated to be at 8240 μM.		* Weighed out .014 g of monohydrate chloroauric acid and dissolved into 5 mL of water. The molarity of gold was calculated to be at 8240 μM.

Mary Mendoza: /* Preparation of Gold and Lysozyme stocks */

2012-10-27T18:32:53Z

Preparation of Gold and Lysozyme stocks

←Older revision		Revision as of 18:32, 27 October 2012
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	==Preparation of Gold and Lysozyme stocks==		==Preparation of Gold and Lysozyme stocks==
-	* Michael Nagle prepared a new set of Au/BSA solutions. However, calculations made are not available in his notebook.	+	* Michael Nagle prepared a new set of Au/BSA solutions. However, calculations made are not available in his notebook. Nagle finished the preparation of the set and placed the rack at 85°C for 4h. at the Thermo Scientific incubator.
	* Weighed out .014 g of monohydrate chloroauric acid and dissolved into 5 mL of water. The molarity of gold was calculated to be at 8240 μM.		* Weighed out .014 g of monohydrate chloroauric acid and dissolved into 5 mL of water. The molarity of gold was calculated to be at 8240 μM.

Mary Mendoza: /* Preparation of Lysozyme stock */

2012-10-27T18:24:27Z

Preparation of Lysozyme stock

←Older revision		Revision as of 18:24, 27 October 2012
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	* Data collection was not possible. No signal was collected.		* Data collection was not possible. No signal was collected.

-	==Preparation of Lysozyme ~~stock~~==	+	==Preparation of Gold and Lysozyme stocks==
	* Michael Nagle prepared a new set of Au/BSA solutions. However, calculations made are not available in his notebook.		* Michael Nagle prepared a new set of Au/BSA solutions. However, calculations made are not available in his notebook.
		+	* Weighed out .014 g of monohydrate chloroauric acid and dissolved into 5 mL of water. The molarity of gold was calculated to be at 8240 μM.
		+
		+
		+	.014 g of gold × <math>\frac{1 mol}{339.79 g}</math> = 4.12E(-5) mol ÷ .005 L = <math>\frac{8.24E(-3)mol}{L}</math> = 8240 μM
		+
		+
	* Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM.		* Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM.

Mary Mendoza: /* Preparation of Lysozyme stock */

2012-10-27T18:04:57Z

Preparation of Lysozyme stock

←Older revision		Revision as of 18:04, 27 October 2012
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	* After preparing the stock, it was decided to prepare the Au/lysozyme solution next week. The clear and colorless, 15.24 μM stock solution of lysozyme was refrigerated.		* After preparing the stock, it was decided to prepare the Au/lysozyme solution next week. The clear and colorless, 15.24 μM stock solution of lysozyme was refrigerated.
		+	* In addition, the Au/lysozyme mole ratios chosen were similar to the 14 Au/BSA solutions. Likewise to the Au/BSA solutions, the lysozyme will be kept constant at a volume of .590 mL = 590 μL.
		+
		+

Mary Mendoza: /* Preparation of Lysozyme stock */

2012-10-27T17:54:10Z

Preparation of Lysozyme stock

←Older revision		Revision as of 17:54, 27 October 2012
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	* Michael Nagle prepared a new set of Au/BSA solutions. However, calculations made are not available in his notebook.		* Michael Nagle prepared a new set of Au/BSA solutions. However, calculations made are not available in his notebook.
	* Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM.		* Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM.
		+

	.012 L of water × <math>\frac{15E(-6)mol}{1 L}</math> = <math>\frac{1.80E(-7)mol}{L}</math> × <math>\frac{14,307 g}{mol}</math> = 0.00258 g of lysozyme to obtain 15 μM		.012 L of water × <math>\frac{15E(-6)mol}{1 L}</math> = <math>\frac{1.80E(-7)mol}{L}</math> × <math>\frac{14,307 g}{mol}</math> = 0.00258 g of lysozyme to obtain 15 μM
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	* The amount to be measured is too small to be certain that the entire solid would be dissolved in water. As a result, a multiple of the calculated amount was chosen to be dissolved in water; the amount chosen was 0.0109 g. Once dissolved, this will be diluted to 15 μM.		* The amount to be measured is too small to be certain that the entire solid would be dissolved in water. As a result, a multiple of the calculated amount was chosen to be dissolved in water; the amount chosen was 0.0109 g. Once dissolved, this will be diluted to 15 μM.
		+

	0.0109 g of lysozyme × <math>\frac{1 mol}{14,307 g}</math> = 7.62E(-7) mol ÷ .050 L of water = 1.524E(-5) M = 15.24 μM		0.0109 g of lysozyme × <math>\frac{1 mol}{14,307 g}</math> = 7.62E(-7) mol ÷ .050 L of water = 1.524E(-5) M = 15.24 μM

		+
		+	* After preparing the stock, it was decided to prepare the Au/lysozyme solution next week. The clear and colorless, 15.24 μM stock solution of lysozyme was refrigerated.

Mary Mendoza: /* Preparation of Lysozyme stock */

2012-10-27T17:51:23Z

Preparation of Lysozyme stock

←Older revision		Revision as of 17:51, 27 October 2012
Line 29:		Line 29:
	* Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM.		* Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM.

-	.012 L of water × <math>\frac{15E(-6)mol}{1 L}</math> = <math>\frac{1.80E(-7)mol}{L}</math> × <math>\frac{14,307 g}{mol}</math> = 0.00258 g of lysozyme	+	.012 L of water × <math>\frac{15E(-6)mol}{1 L}</math> = <math>\frac{1.80E(-7)mol}{L}</math> × <math>\frac{14,307 g}{mol}</math> = 0.00258 g of lysozyme to obtain 15 μM
		+
		+
		+	* The amount to be measured is too small to be certain that the entire solid would be dissolved in water. As a result, a multiple of the calculated amount was chosen to be dissolved in water; the amount chosen was 0.0109 g. Once dissolved, this will be diluted to 15 μM.
		+
		+	0.0109 g of lysozyme × <math>\frac{1 mol}{14,307 g}</math> = 7.62E(-7) mol ÷ .050 L of water = 1.524E(-5) M = 15.24 μM
		+
		+

Mary Mendoza: /* Preparation of Lysozyme stock */

2012-10-27T17:45:17Z

Preparation of Lysozyme stock

←Older revision		Revision as of 17:45, 27 October 2012
Line 29:		Line 29:
	* Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM.		* Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM.

-	.012 L of water × <math>\frac{15E(-6)mol}{1 L}</math> = <math>\frac{1.80E(-7)mol}{L}</math>	+	.012 L of water × <math>\frac{15E(-6)mol}{1 L}</math> = <math>\frac{1.80E(-7)mol}{L}</math> × <math>\frac{14,307 g}{mol}</math> = 0.00258 g of lysozyme

Mary Mendoza: /* Preparation of Lysozyme stock */

2012-10-27T17:43:37Z

Preparation of Lysozyme stock

←Older revision		Revision as of 17:43, 27 October 2012
Line 29:		Line 29:
	* Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM.		* Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM.

-	.012 L of water × <math>\frac{15E(-6)~~\mu~~ mol}{1 L}</math>	+	.012 L of water × <math>\frac{15E(-6)mol}{1 L}</math> = <math>\frac{1.80E(-7)mol}{L}</math>

Mary Mendoza: /* Preparation of Lysozyme stock */

2012-10-27T17:40:55Z

Preparation of Lysozyme stock

←Older revision		Revision as of 17:40, 27 October 2012
Line 29:		Line 29:
	* Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM.		* Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM.

-	.012 L of water × <math>\frac{15E(-6)\ mu mol}{1 L}</math>	+	.012 L of water × <math>\frac{15E(-6)\mu mol}{1 L}</math>

Mary Mendoza: /* Preparation of Lysozyme stock */

2012-10-27T17:40:40Z

Preparation of Lysozyme stock

←Older revision		Revision as of 17:40, 27 October 2012
Line 28:		Line 28:
	* Michael Nagle prepared a new set of Au/BSA solutions. However, calculations made are not available in his notebook.		* Michael Nagle prepared a new set of Au/BSA solutions. However, calculations made are not available in his notebook.
	* Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM.		* Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM.
-		+
		+	.012 L of water × <math>\frac{15E(-6)\ mu mol}{1 L}</math>