User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17: Difference between revisions
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* Data collection was not possible. No signal was collected. | * Data collection was not possible. No signal was collected. | ||
==Preparation of Lysozyme | ==Preparation of Gold and Lysozyme stocks== | ||
* Michael Nagle prepared a new set of Au/BSA solutions. However, calculations made are not available in his notebook. | * Michael Nagle prepared a new set of Au/BSA solutions. However, calculations made are not available in his notebook. | ||
* Weighed out .014 g of monohydrate chloroauric acid and dissolved into 5 mL of water. The molarity of gold was calculated to be at 8240 μM. | |||
.014 g of gold × <math>\frac{1 mol}{339.79 g}</math> = 4.12E(-5) mol ÷ .005 L = <math>\frac{8.24E(-3)mol}{L}</math> = 8240 μM | |||
* Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM. | * Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM. | ||
Revision as of 11:24, 27 October 2012
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Electrophoresis of the mutated DNA plasmids
UV-Vis for Chemiluminescence
Preparation of Gold and Lysozyme stocks
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