User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17: Difference between revisions
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* Michael Nagle prepared a new set of Au/BSA solutions. However, calculations made are not available in his notebook. | * Michael Nagle prepared a new set of Au/BSA solutions. However, calculations made are not available in his notebook. | ||
* Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM. | * Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM. | ||
.012 L of water × <math>\frac{15E(-6)mol}{1 L}</math> = <math>\frac{1.80E(-7)mol}{L}</math> × <math>\frac{14,307 g}{mol}</math> = 0.00258 g of lysozyme to obtain 15 μM | .012 L of water × <math>\frac{15E(-6)mol}{1 L}</math> = <math>\frac{1.80E(-7)mol}{L}</math> × <math>\frac{14,307 g}{mol}</math> = 0.00258 g of lysozyme to obtain 15 μM | ||
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* The amount to be measured is too small to be certain that the entire solid would be dissolved in water. As a result, a multiple of the calculated amount was chosen to be dissolved in water; the amount chosen was 0.0109 g. Once dissolved, this will be diluted to 15 μM. | * The amount to be measured is too small to be certain that the entire solid would be dissolved in water. As a result, a multiple of the calculated amount was chosen to be dissolved in water; the amount chosen was 0.0109 g. Once dissolved, this will be diluted to 15 μM. | ||
0.0109 g of lysozyme × <math>\frac{1 mol}{14,307 g}</math> = 7.62E(-7) mol ÷ .050 L of water = 1.524E(-5) M = 15.24 μM | 0.0109 g of lysozyme × <math>\frac{1 mol}{14,307 g}</math> = 7.62E(-7) mol ÷ .050 L of water = 1.524E(-5) M = 15.24 μM | ||
* After preparing the stock, it was decided to prepare the Au/lysozyme solution next week. The clear and colorless, 15.24 μM stock solution of lysozyme was refrigerated. | |||
Revision as of 10:54, 27 October 2012
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Electrophoresis of the mutated DNA plasmids
UV-Vis for Chemiluminescence
Preparation of Lysozyme stock
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