User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Mary Mendoza (talk | contribs) |
Mary Mendoza (talk | contribs) |
||
Line 18: | Line 18: | ||
* Completion of the electrophoresis was followed by the addition of ethidium bromide (EtBr). Great caution was done in handling EtBr since this agent is carcinogenic by binding to any DNA in contact. | * Completion of the electrophoresis was followed by the addition of ethidium bromide (EtBr). Great caution was done in handling EtBr since this agent is carcinogenic by binding to any DNA in contact. | ||
* 20 μL of the 12.3 g/mL of EtBR was added to the gel suspended in TAE placed on a microwaveable plastic container. This was stirred in a VWR analog shaker at a slow speed. | * 20 μL of the 12.3 g/mL of EtBR was added to the gel suspended in TAE placed on a microwaveable plastic container. This was stirred in a VWR analog shaker at a slow speed. | ||
* Assuming that the gel has absorbed the EtBr, the gel was placed on top of a UV lamp. Only the DNA ladded inserted on the left most part of the gel exhibited orange fluorescence. | |||
* The orange fluorescence is attributed to the EtBr binding to the DNA. | |||
==UV-Vis for Chemiluminescence== | ==UV-Vis for Chemiluminescence== |
Revision as of 10:33, 27 October 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Electrophoresis of the mutated DNA plasmids
UV-Vis for Chemiluminescence
Preparation of Lysozyme stock
|