# User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/16

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## PCR mutation

• In reference to the PCR Mutation protocol, 100 ng/μL of the primer was needed for the reaction. The weight of the primer in the provided container was 0.46 mg. A ratio of the weight over volume was equated to the required concentration of the primer:

0.46 mg = 0.46E6 ng

$\frac{0.46E6 ng}{x \mu L}$ = $\frac{100 ng}{1 \mu L}$