User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/16: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Mary Mendoza (talk | contribs) |
Mary Mendoza (talk | contribs) |
||
(7 intermediate revisions by the same user not shown) | |||
Line 12: | Line 12: | ||
<math>\frac{0.46E6 ng}{x \mu L}</math> = <math>\frac{100 ng}{1 \mu L}</math> of primer in water = 4600 μL of water | <math>\frac{0.46E6 ng}{x \mu L}</math> = <math>\frac{100 ng}{1 \mu L}</math> of primer in water = 4600 μL of water | ||
Line 22: | Line 22: | ||
* The procedure listed in [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol|PCR Mutation protocol]] was strictly followed. After the addition of all reagents, the sample was placed in the thermocycler. | * The procedure listed in [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol|PCR Mutation protocol]] was strictly followed. After the addition of all reagents, the sample was placed in the thermocycler. The amplified DNA was contained in a sterilized, 1.5 microcentrifuge tube. | ||
==Continuation of Chemiluminescence== | ==Continuation of Chemiluminescence== | ||
Line 43: | Line 43: | ||
* Due to time constraints, the chemiluminescence of the luminol at pH 10.55 was tested on a lab bench with the room lights turned off. The reaction produced a neon blue glow that lasted for more than 3 minutes. No photograph was taken since the apparatus of the camera was not suited for the fluorescence activity. | * Due to time constraints, the chemiluminescence of the luminol at pH 10.55 was tested on a lab bench with the room lights turned off. The reaction produced a neon blue glow that lasted for more than 3 minutes. No photograph was taken since the apparatus of the camera was not suited for the fluorescence activity. | ||
* The volume of each reagent added to the cuvette are listed below. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''' | |||
| align="center" style="background:#f0f0f0;"|''HRP'' | |||
| align="center" style="background:#f0f0f0;"|''4-iodophenol'' | |||
| align="center" style="background:#f0f0f0;"|''luminol'' | |||
| align="center" style="background:#f0f0f0;"|''H<sub>2</sub>O<sub>2</sub>'' | |||
| align="center" style="background:#f0f0f0;"|''H<sub>2</sub>O'' | |||
|- | |||
| Molarity of stock solutions||2.3 μM||18 mM||10 mM||1.7 mM||neutral | |||
|- | |||
| Volume||33 μL||27 μL||450 μL||88 μL||902 μL | |||
|- | |||
Revision as of 07:00, 7 December 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||
PCR mutation
0.46 mg = 0.46E6 ng
Continuation of Chemiluminescence
[math]\displaystyle{ \frac{1.91 g}{15 mL} }[/math] × [math]\displaystyle{ \frac{1 mol}{105.9784 g} }[/math] = [math]\displaystyle{ \frac{0.00120 mol}{mL} }[/math] × [math]\displaystyle{ \frac{1 mL}{1E(-3) L} }[/math] = [math]\displaystyle{ \frac{1.20 mol}{L} }[/math] = 1.20 M of sodium carbonate
|