User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/16: Difference between revisions
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* There is limited space in the plastic container (1 mL). Instead of dissolving 0.46E6 ng of the primer in 4600 μL of water, the entire primer was dissolved in 1 mL water. | * There is limited space in the plastic container (1 mL). Instead of dissolving 0.46E6 ng of the primer in 4600 μL of water, the entire primer was dissolved in 1 mL water. | ||
* Using M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>, the volume taken from the solution of 0.46E6 ng in 1 mL of water was calculated to be 217.39 μL. This was transferred to a new tube and filled up with water to a total volume of 1 mL. | * Using M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>, the volume taken from the solution of 0.46E6 ng in 1 mL of water was calculated to be 217.39 μL. This was transferred to a new tube and filled up with water to a total volume of 1 mL. | ||
V<sub>1</sub> = <math>\frac{100 ng/ \mu L * 1000 \mu L}{0.46E3 ng/ \mu L}</math> = 217.39 μL of the dissolved primer in water | V<sub>1</sub> = <math>\frac{100 ng/ \mu L * 1000 \mu L}{0.46E3 ng/ \mu L}</math> = 217.39 μL of the dissolved primer in water |
Revision as of 22:55, 25 October 2012
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PCR mutation
0.46 mg = 0.46E6 ng
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