# User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/16

(Difference between revisions)
 Revision as of 01:27, 26 October 2012 (view source) (→PCR mutation)← Previous diff Revision as of 01:31, 26 October 2012 (view source) (→PCR mutation)Next diff → Line 7: Line 7: ==PCR mutation== ==PCR mutation== - * In reference to the [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol|PCR Mutation protocol]], 100 ng/μL of the primer was needed for the reaction. The weight of the primer in the provided container was [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/10|0.46 mg.]] + * In reference to the [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol|PCR Mutation protocol]], 100 ng/μL of the primer was needed for the reaction. The weight of the primer in the provided container was [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/10|0.46 mg.]] A ratio of the weight over volume was equated to the required concentration of the primer: + + 0.46 mg = 0.46E6 ng + + + $\frac{0.46E6 ng}{x μL}$ = $\frac{100 ng}{1 μL}$

## Revision as of 01:31, 26 October 2012

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## PCR mutation

• In reference to the PCR Mutation protocol, 100 ng/μL of the primer was needed for the reaction. The weight of the primer in the provided container was 0.46 mg. A ratio of the weight over volume was equated to the required concentration of the primer:

0.46 mg = 0.46E6 ng

$\frac{0.46E6 ng}{x μL}$ = $\frac{100 ng}{1 μL}$