User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/09: Difference between revisions
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* The actual of the yellow pastel powder of luminol transferred in 2 mL of water was 0.0108 g. Luminol was insoluble in water. Hence, 0.0729 g of the white granular solid of sodium carbonate was added into solution. The solution of luminol, sodium carbonate in 2 mL water was vortexed for faster dissolution. | * The actual amount of the yellow pastel powder of luminol transferred in 2 mL of water was 0.0108 g. Luminol was insoluble in water. Hence, 0.0729 g of the white granular solid of sodium carbonate was added into solution. The solution of luminol, sodium carbonate in 2 mL water was vortexed for faster dissolution. | ||
* To buffer the alkalinity of sodium carbonate, the amount of 0.4373 g of sodium bicarbonate was added. | |||
* Sodium bicarbonate precipitated in the solution. Addition of 4 mL of water diluted luminol from 30 mM to 10 mM. | |||
* A pH paper was used to estimate the pH level of the solution since the electrode of the pH meter did not fit into the falcon tube of the solution. | |||
==Fluorescence Spectrometer Graph== | ==Fluorescence Spectrometer Graph== |
Revision as of 19:56, 25 October 2012
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Atomic Absorption Spectroscopy of Au/BSA
Chemiluminescence of Luminol
Fluorescence Spectrometer GraphNo luminescence was observed for any runs. The concentration of reagents are shown below the table:
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