User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/09: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Mary Mendoza (talk | contribs) |
Mary Mendoza (talk | contribs) |
||
(4 intermediate revisions by the same user not shown) | |||
Line 53: | Line 53: | ||
Image 2. Below each pH paper is shown the number of drops of 1 M HCl into the solution of luminol. The light green color corresponds to the pH level of 7 to 8. | Image 2. Below each pH paper is shown the number of drops of 1 M HCl into the solution of luminol. The light green color corresponds to the pH level of 7 to 8. | ||
* The lamp of LS 55 Perkin Elmer fluorescence spectrometer was turned off. The signal was set to a function of time. The wavelength was set at 425 nm. The excitation slit was placed at 15 nm while the emission slit was placed at 20 nm. The duration of data collection was settled at 300 sec. | |||
* The reagents were transferred into the cuvette using automatic delivery micropipets. The HRP was added last for this initiates the reaction through catalysis of hydrogen peroxide. | |||
* The collected data are shown in the next section. | |||
==Fluorescence Spectrometer Graph== | ==Fluorescence Spectrometer Graph== | ||
Line 62: | Line 67: | ||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'' | | align="center" style="background:#f0f0f0;"|'''' | ||
| align="center" style="background:#f0f0f0;"|''HRP'' | |||
| align="center" style="background:#f0f0f0;"|''4-iodophenol'' | | align="center" style="background:#f0f0f0;"|''4-iodophenol'' | ||
| align="center" style="background:#f0f0f0;"|''luminol'' | | align="center" style="background:#f0f0f0;"|''luminol'' | ||
Line 68: | Line 74: | ||
| align="center" style="background:#f0f0f0;"|''H<sub>2</sub>O'' | | align="center" style="background:#f0f0f0;"|''H<sub>2</sub>O'' | ||
|- | |- | ||
| 1||30 μL||900 μL||176 μL||1.8 mL | | Molarity of Stock Solutions||2.3 μM||18 mM||10 mM||1.7 mM||neutral | ||
|- | |||
| Volume||30 μL||54 μL||900 μL||176 μL||1.8 mL | |||
|- | |- | ||
Revision as of 06:56, 7 December 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||
Atomic Absorption Spectroscopy of Au/BSA
Chemiluminescence of Luminol
Image 1. The navy blue color is attributed to a pH of 10 to 11. Image 2. Below each pH paper is shown the number of drops of 1 M HCl into the solution of luminol. The light green color corresponds to the pH level of 7 to 8.
Fluorescence Spectrometer GraphNo luminescence was observed for any runs. The concentration of reagents are shown below the table:
|